Ephic (Electrophysiology and Imaging with C++)

• Introduction
• Features
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• Tutorials
• Notes

Tutorial #2

• Electrophysiology Window
• Electrophysiology Controls
• Command waveform: Pulse

Electrophysiology Window

The electrophysiology window displays the command (light green), the current trace (blue), the voltage trace (red), and the extracellular stimulator command (cyan). The voltage scale is on the left. The current scale is on the right. The time scale is on the bottom. To move the voltage or the current trace up or down, click and hold the left mouse button at the respective scale and drag up or down. To move traces left or right, click and hold the left mouse button at the time sacle and drag left or right. To change the voltage scale or the current scale, click and hold the right mouse button at the respective scale and drag away from or closer to the reference line. To change the time scale, click and hold the right mouse button at the time scale and drag away from or closer to the left border line. To reset the electrophysiology window, press and hold "Ctrl" and click the left mouse button inside the window.

Electrophysiology Controls

In this example, Ephic is in  the "V Clamp" mode. To change to the "I Clamp" mode, click the "I Clamp" button. There are five basic waveforms for command: Pulse, Alpha, Chirp, Ramp, and Sine. In this example, the Pulse waveform is used. With this default settings, Ephic calculates the resistance and displays it in "Rin (MOhm)" box.

Command Waveform: Pulse

In this example, Ephic is in the "I Clamp" mode. The command waveform is Pulse. The amplitude is 0.18 nA. The pulse starts at 100 ms. The width of the pulse is 30 ms. There is only one pulse in a burst and one burst in the command. Thus, the interval between pulses and the interval between bursts do not have any effect on the command. The figure on the right displays the command waveform and the voltage response from a mouse CA1 pyramidal neuron.
This is a pulse command waveform during a TBP experiment. For each pulse, the amplitude is 2 nA. The first pulse starts at 205 ms. The width of pulse is 1 ms. There are 5 pulses in each burst and the interval between two pulses is 10 ms. There are 10 bursts and the interval between two bursts is 200 ms.